FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - CaMK1A (2 - 369)

Name: CaMK1A (2 - 369)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1148
Accession:
NM_003656
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 500 μM CaCl2, 0.3 μM calmodulin, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM magnesium acetate
Enzymatic Substrate:
YLRRRLSDSNF-amide [Residues 3 - 13 of Synapsin 1]; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 67,986.3
Average Mass: 68,030.07
Protein Activity:
Constitutively Active
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-20 °C
Theoretical pI:
5.31
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids L2 – L369 (end) of human CaMK1. Residue L232 of the fusion protein is equivalent to L2 of the native enzyme. The GST tag is located at residues 1 - 220
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and EcoR1 site of pGEX 6P-1

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