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Recombinant Protein - CaMK1A (2 - 369)
Name: CaMK1A (2 - 369)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 500 μM CaCl2, 0.3 μM calmodulin, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM magnesium acetate
YLRRRLSDSNF-amide [Residues 3 - 13 of Synapsin 1]; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 67,986.3
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Amino acids L2 – L369 (end) of human CaMK1. Residue L232 of the fusion protein is equivalent to L2 of the native enzyme. The GST tag is located at residues 1 - 220
PreScission (LEVLFQGPL) residues 221 - 229
BamH1 and EcoR1 site of pGEX 6P-1
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