FESTIVE CLOSURE

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Recombinant Protein - CAK1 (2 - 368)

Name: CAK1 (2 - 368)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1089
Accession:
U60192
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

C-Term
6His
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Two step assay in which CAK1 activates CDK2 [DU 1043] / cyclin A2 [DU 1064]. The activity of CDK2 / cyclin A2 is then assayed against Histone H1 as a substrate (final concentration of 1 mg/ml) in the standard filter binding assay.
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0. 1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.1 mM sodium vanadate, 10 mM magnesium acetate
Enzymatic Substrate:
Histone H1
Calculated molecular mass:
Mono-Isotopic Mass: 69,801.22
Average Mass: 69,846.38
Protein Activity:
Constitutively Active
Purity:
70 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
5.88
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids K2 – P368 (end) of yeast CAK1. Residue K232 of fusion protein is equilivalent to K2 of the native enzyme. The GST tag is located at residues 1 - 220 and the His(6) tag is located at residues 601 – 606. The following sequence is present after the CAK1 sequence and before the His(6) tag, GS, residues 599 and 600.
Protease Cleavage:
PreScission (LEVLFQGPL) at residues 221 – 229
Cloning Sites:
BamH1 and EcoR1 site of pGEX6P-1

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