Two step assay in which CAK1 activates CDK2 [DU 1043] / cyclin A2 [DU 1064]. The activity of CDK2 / cyclin A2 is then assayed against Histone H1 as a substrate (final concentration of 1 mg/ml) in the standard filter binding assay.
50 mM Tris-HCl pH 7.5, 0. 1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.1 mM sodium vanadate, 10 mM magnesium acetate
Calculated molecular mass:
Mono-Isotopic Mass: 69,801.22
Average Mass: 69,846.38
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Gel Information :
Gel Image 1:
Amino acids K2 – P368 (end) of yeast CAK1. Residue K232 of fusion protein is equilivalent to K2 of the native enzyme. The GST tag is located at residues 1 - 220 and the His(6) tag is located at residues 601 – 606. The following sequence is present after the CAK1 sequence and before the His(6) tag, GS, residues 599 and 600.
PreScission (LEVLFQGPL) at residues 221 – 229
BamH1 and EcoR1 site of pGEX6P-1
Have a Question?
If you would like any assistance with your order, please don’t hesitate to get in touch.