FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - BTK (2 - 659)

Name: BTK (2 - 659)
Price per aliquot (100µg): £100.00
Long Name:
Bruton agammaglobulinemia Tyrosine Kinase
Catalog Number (DU Number):
DU12110
Accession:
NP_00052.1
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
KVEKIGEGTYGVVYK; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 79,470.73
Average Mass: 79,521.68
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
7.06
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – S659 (end) of human BTK. Residue A29 of the fusion protein is equivalent to A2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Not1 sites of pFastBAC HTb

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