FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - BRSK2 (2 - 674) *

Name: BRSK2 (2 - 674) *
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1280
Accession:
AF533878
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Generic peptide [KKLNRTLSFAEPG]; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 78,340.53
Average Mass: 78,389.58
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
8.84
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – L674 (end) of human BRSK2. Residue T29 of fusion protein is equilivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 site of pFastBAC HTb

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