Recombinant Protein - BRSK1 (2 - 778) *

Name: BRSK1 (2 - 778) *
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1270
Accession:
NM_032430
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+ NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1% 2-mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
CHKtide [KKKVSRSGLYRSPSMPENLNRPR]; Final concentration: 300μM
Calculated molecular mass:
Mono-Isotopic Mass: 88,272.15
Average Mass: 88,327.19
Protein Activity:
Constitutively Active
Purity:
>80%
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCI, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
9.24
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2- P778 (end) of human BRSKl. Residue S29 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5- 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18- 24
Cloning Sites:
BamH1 sites of pFastBAC HTb

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