FESTIVE CLOSURE

Festive Closure

We will be closed between 21st December to 4th January. Orders may be placed during this time but will not be shipped until January 2018. Seasons Greetings to all our customers.

Recombinant Protein - Aurora C (2 - 309)

Name: Aurora C (2 - 309)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU4009
Accession:
ABO17332
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 0.1 mM sodium vanadate, 10 mM magnesium acetate
Enzymatic Substrate:
LRRLSLGLRRLSLGLRRLSLGLRRLSLG; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 38,791.10
Average Mass: 38,815.66
Notes:
Following expression the culture is incubated with 50 nM okadaic acid for 1 hour prior to purification
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 8, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
8.46
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – S309 (end) of human Aurora C. Residue S29 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10. The following amino acid substitution is present: L – P, where L301 of the native enzyme is P328 of the fusion protein
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Sal1 sites of pFastBAC HTb

Do you need any help? Please get in touch and we’ll be happy to lend a hand.