Name: Aurora C (2 - 309)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 0.1 mM sodium vanadate, 10 mM magnesium acetate
LRRLSLGLRRLSLGLRRLSLGLRRLSLG; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 38,791.10
Following expression the culture is incubated with 50 nM okadaic acid for 1 hour prior to purification
50 mM Tris-HCl pH 8, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
-70 °C [Long term stability to be determined]
Amino acids S2 – S309 (end) of human Aurora C. Residue S29 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
The following amino acid substitution is present: L – P, where L301 of the native enzyme is P328 of the fusion protein
rTEV (ENLYFQG) residues 18 - 24
BamH1 and Sal1 sites of pFastBAC HTb