Recombinant Protein - Aurora C (2 - 309)

Name: Aurora C (2 - 309)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU4009
Accession:
ABO17332
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 0.1 mM sodium vanadate, 10 mM magnesium acetate
Enzymatic Substrate:
LRRLSLGLRRLSLGLRRLSLGLRRLSLG; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 38,791.10
Average Mass: 38,815.66
Notes:
Following expression the culture is incubated with 50 nM okadaic acid for 1 hour prior to purification
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 8, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
8.46
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S2 – S309 (end) of human Aurora C. Residue S29 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10. The following amino acid substitution is present: L – P, where L301 of the native enzyme is P328 of the fusion protein
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Sal1 sites of pFastBAC HTb

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