FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - Aurora B (1 - 344)

Name: Aurora B (1 - 344)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU1773
Accession:
NM_004217
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2 mercaptoethanol, 0.1 mM EGTA, 0.1 mM sodium vanadate, 10 mM magnesium acetate
Enzymatic Substrate:
LRRLSLGLRRLSLGLRRLSLGLRRLSLG; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 40,208.97
Average Mass: 40,234.49
Notes:
Following expression the culture is incubated with 50 nM okadaic acid for 1 hour prior to purification
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 8, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical pI:
9.36
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – A344 (end) of human Aurora B. Residue M8 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 2 – 7.
Protease Cleavage:
None
Cloning Sites:
Nde1 and Xho1 sites of modified pFastBAC 1

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