Recombinant Protein - Aurora A (2 - 403)
Name: Aurora A (2 - 403)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
LRRLSLGLRRLSLGLRRLSLGLRRLSLG; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 49,191.32
Has been Activated in Vitro
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
-70 °C [Long term stability to be determined]
Amino acids D2 – S403 (end) of human Aurora A. Residue D35 of the fusion protein is equivalent to D2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Thrombin (LVPRGS) at residues 14 - 19
BamH1 and Sal1 sites of pET28a(+)
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