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Recombinant Protein - Aurora A (2 - 403)

Name: Aurora A (2 - 403)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU4174
Accession:
BC027464
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
LRRLSLGLRRLSLGLRRLSLGLRRLSLG; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 49,191.32
Average Mass: 49,222.02
Protein Activity:
Has been Activated in Vitro
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C [Long term stability to be determined]
Theoretical PI:
9.51
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids D2 – S403 (end) of human Aurora A. Residue D35 of the fusion protein is equivalent to D2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
Thrombin (LVPRGS) at residues 14 - 19
Cloning Sites:
BamH1 and Sal1 sites of pET28a(+)

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