Name: AMPK alpha1 catalytic subunit [T172D] (3 - 308)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
AMARA peptide [AMARAASAAALARRR]; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 64,554.94
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine
Amino acids E3 - L308 of rat AMP kinase alpha 1. [Full length protein ends at residue Q548]
Residue E244 of the fusion protein is equivalent to E3 of the native enzyme.
The enzyme has a T172D mutation in order to mimic phosphorylation of the enzyme. Residue T172 is equilivalent to D413 of the fusion protein.
The GST tag is located at residues 1 - 220 and the MYC tag is located at residues 231 - 240.
The following sequence is present after the AMPK sequence, RSITLAAARDRLD, residues 550 - 562.
Thrombin (LVPRGS) residues 221 - 226
BamH1 and Not1 of pGEX 4T