Recombinant Protein - AMPK alpha1 catalytic subunit [T172D] (3 - 308)

Name: AMPK alpha1 catalytic subunit [T172D] (3 - 308)
Price per aliquot (100µg): £100.00
Synonyms:
AMPKA
Catalog Number (DU Number):
DU1713
Accession:
U40819
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved

N-Term
Myc
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
AMARA peptide [AMARAASAAALARRR]; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 64,554.94
Average Mass: 64,596.68
Protein Activity:
Constitutively Active
Purity:
> 90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
-20 °C
Theoretical pI:
6.05
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids E3 - L308 of rat AMP kinase alpha 1. [Full length protein ends at residue Q548] Residue E244 of the fusion protein is equivalent to E3 of the native enzyme. The enzyme has a T172D mutation in order to mimic phosphorylation of the enzyme. Residue T172 is equilivalent to D413 of the fusion protein. The GST tag is located at residues 1 - 220 and the MYC tag is located at residues 231 - 240. The following sequence is present after the AMPK sequence, RSITLAAARDRLD, residues 550 - 562.
Protease Cleavage:
Thrombin (LVPRGS) residues 221 - 226
Cloning Sites:
BamH1 and Not1 of pGEX 4T

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