Synonyms
AF6; MLL-AF6; MLLT4; AFDN
Plasmid
pcDNA5 FRT/TO 3FLAG Afadin (MLLT4)
Parent Plasmid
pcDNA5 FRT/TO 3FLAG
Expressed
3FLAG Afadin (MLLT4)
Genbank
Codon optimised XM_005266996.3
Tag
3FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCATGTCTGCTGGCGGAAGAGATGAGGAACGCAGAAAGCTGGCCGACATCATCCACCACTGGAACGCCAACAGACTGGACCTGTTCGAGATCAGCCAGCCTACCGAGGACCTGGAATTTCACGGCGTGATGCGGTTCTACTTCCAAGACAAGGCCGCTGGCAACTTCGCCACCAAGTGCATCAGAGTGTCCAGCACCGCCACCACACAGGACGTGATCGAAACACTGGCCGAGAAGTTCAGACCCGACATGCGGATGCTGAGCAGCCCCAAGTACAGCCTGTATGAGGTGCACGTGTCCGGCGAGAGAAGGCTGGACATCGATGAGAAGCCTCTGGTGGTGCAGCTGAACTGGAACAAGGACGACCGCGAGGGCAGATTCGTGCTGAAGAACGAGAACGACGCCATTCCTCCTAAGAAGGCCCAGAGCAACGGCCCCGAGAAGCAAGAGAAAGAAGGCGTGATCCAGAACTTCAAGCGGACCCTGTCCAAGAAAGAGAAAAAAGAGAAGAAGAAGCGCGAGAAAGAGGCCCTGAGACAGGCCAGCGACAAGGATGACAGACCCTTCCAGGGCGAAGATGTGGAAAACAGCAGACTGGCCGCCGAGGTGTACAAGGACATGCCCGAGACAAGCTTCACCCGGACCATCAGCAATCCCGAGGTGGTCATGAAGCGGCGGAGACAGCAGAAACTGGAAAAGCGGATGCAAGAGTTCAGAAGCAGCGACGGCAGACCTGATTCTGGCGGCACCCTGAGAATCTACGCCGACAGCCTGAAGCCTAACATCCCCTACAAGACCATCCTGCTGAGCACCACCGATCCTGCCGATTTTGCCGTGGCCGAGGCTCTCGAGAAGTACGGCCTGGAAAAAGAAAACCCCAAGGACTACTGTATCGCCCGCGTGATGTTGCCTCCTGGCGCTCAGCATTCTGATGAGAAGGGCGCCAAAGAGATCATCCTGGACGACGACGAGTGCCCTCTGCAGATCTTTAGAGAGTGGCCCTCCGACAAGGGCATCCTGGTGTTCCAGCTGAAGAGGCGGCCTCCTGATCACATCCCTAAAAAGACCAAGAAGCACCTCGAGGGCAAGACCCCTAAGGGCAAAGAAAGAGCCGACGGCAGCGGCTACGGAAGCACACTGCCTCCAGAGAAGCTGCCCTACCTGGTGGAACTGAGCCCCGGCAGAAGAAACCACTTCGCCTACTACAACTACCACACCTACGAGGACGGCTCCGACAGCAGAGACAAGCCCAAGCTGTACAGACTGCAGCTGAGCGTGACCGAAGTGGGCACCGAGAAGCTGGACGACAACAGCATCCAGCTGTTCGGCCCTGGAATCCAGCCTCACCACTGCGACCTGACCAACATGGATGGCGTGGTCACAGTGACCCCTAGATCCATGGACGCCGAGACATACGTGGAAGGCCAGCGGATCAGCGAGACAACCATGCTGCAGAGCGGCATGAAGGTGCAGTTTGGCGCCAGCCACGTGTTCAAGTTCGTGGACCCTAGCCAGGATCATGCCCTGGCCAAGAGATCTGTGGATGGCGGCCTGATGGTCAAGGGCCCTAGACACAAGCCTGGCATCGTGCAAGAGACAACCTTCGATCTCGGCGGCGATATCCACAGCGGAACAGCCCTGCCTACCAGCAAGAGCACCACAAGACTGGACAGCGACAGAGTGTCTAGCGCCAGCTCTACAGCCGAGAGAGGCATGGTCAAGCCCATGATCAGAGTGGAACAGCAGCCCGACTACCGGCGCCAAGAGTCTAGAACTCAGGATGCCTCTGGACCCGAGCTGATCCTGCCTGCCTCCATCGAGTTTAGAGAGAGCAGCGAGGACAGCTTCCTGTCCGCCATCATCAACTACACCAACAGCAGCACCGTGCACTTCAAGCTGAGCCCCACCTACGTGCTGTACATGGCCTGTAGATACGTGCTGAGCAACCAGTACAGGCCCGACATCAGCCCTACCGAGAGAACCCACAAAGTGATCGCCGTGGTCAACAAGATGGTGTCCATGATGGAAGGGGTCATCCAGAAGCAGAAGAATATCGCTGGCGCCCTGGCCTTTTGGATGGCCAATGCAAGCGAGCTGCTGAACTTCATCAAGCAGGACCGGGACCTGAGCCGGATCACACTGGATGCTCAGGATGTGCTGGCCCACCTGGTGCAGATGGCCTTCAAGTATCTGGTGCACTGCCTGCAGTCCGAGCTGAACAACTACATGCCCGCCTTTCTGGACGACCCTGAAGAGAACTCACTGCAGCGGCCCAAGATCGACGATGTGCTGCATACACTGACAGGCGCCATGAGCCTGCTGCGGAGATGCAGAGTTAATGCTGCCCTGACCATTCAGCTGTTCAGCCAGCTGTTTCACTTTATCAACATGTGGCTGTTCAACCGGCTGGTCACAGACCCTGATAGCGGCCTGTGTAGCCACTATTGGGGAGCCATCATTCGGCAGCAGCTGGGACACATTGAAGCCTGGGCCGAGAAACAGGGACTCGAGCTGGCTGCCGATTGCCACCTGTCTAGAATCGTGCAGGCCACCACTCTGCTGACCATGGATAAGTACGCCCCTGACGACATCCCCAACATCAACAGCACCTGTTTTAAGCTGAATAGCCTCCAGCTGCAGGCCCTGCTGCAGAACTATCACTGTGCCCCAGACGAGCCTTTCATCCCCACCGACCTGATCGAGAACGTCGTGACCGTGGCTGAGAACACCGCCGATGAACTGGCCAGATCCGATGGCAGAGAGGTGCAGCTCGAGGAAGATCCCGATCTGCAGCTGCCTTTCCTGCTGCCTGAGGATGGCTACAGCTGTGACGTCGTGCGGAATATCCCCAACGGCCTGCAAGAATTCCTGGACCCTCTGTGCCAGCGGGGCTTCTGCAGACTGATCCCTCACACAAGAAGCCCTGGCACCTGGACCATCTACTTCGAGGGCGCCGATTACGAGAGCCATCTGCTGAGAGAGAACACAGAGCTGGCCCAGCCTCTGAGAAAAGAGCCCGAGATCATCACCGTGACACTGAAGAAACAGAACGGCATGGGCCTGTCCATCGTGGCCGCTAAAGGTGCCGGACAGGATAAGCTGGGCATCTACGTGAAGTCCGTCGTGAAAGGCGGAGCCGCCGATGTTGATGGTAGACTGGCTGCTGGCGATCAGCTGCTTTCCGTGGATGGCAGATCTCTCGTGGGCCTCAGCCAAGAAAGGGCCGCAGAGCTGATGACCAGAACCAGCAGCGTTGTGACCCTGGAAGTGGCCAAACAGGGCGCAATCTATCACGGCCTGGCCACACTGCTGAACCAGCCATCTCCTATGATGCAGAGAATCAGCGATCGGAGAGGCAGCGGCAAGCCCAGACCTAAGTCTGAGGGCTTCGAGCTGTACAACAATAGCACCCAGAACGGCTCCCCAGAGAGCCCTCAACTTCCTTGGGCCGAGTACAGCGAGCCCAAAAAACTGCCCGGCGACGACCGGCTGATGAAGAACAGAGCTGACCACCGCAGCAGCCCTAACGTGGCAAATCAGCCTCCATCTCCTGGCGGCAAAAGCGCCTATGCCTCCGGAACAACCGCCAAGATCACCAGCGTGTCCACCGGCAATCTGTGCACCGAGGAACAGACCCCTCCACCTAGACCTGAGGCTTACCCCATTCCTACACAGACCTACACCAGAGAGTACTTCACATTCCCCGCCAGCAAGTCCCAGGACAGAATGGCCCCTCCACAGAACCAGTGGCCTAACTACGAAGAGAAGCCCCACATGCACACCGACAGCAACCACAGCTCTATCGCCATCCAGAGAGTGACCAGAAGCCAAGAGGAACTGAGAGAGGACAAGGCTTACCAGCTGGAAAGACACAGAATCGAAGCCGCCATGGACCGGAAGTCCGACTCCGACATGTGGATCAACCAGAGCAGCAGCCTGGACAGCTCCACCAGCTCTCAAGAACACCTGAACCACTCCTCCAAGAGCGTGACACCAGCCAGCACACTGACCAAGTCTGGACCTGGCAGATGGAAAACCCCTGCCGCCATTCCAGCTACACCTGTGGCTGTGTCTCAGCCCATCAGAACCGACCTTCCACCTCCTCCGCCTCCACCTCCAGTTCATTACGCCGGCGATTTCGACGGCATGAGCATGGATTTGCCCCTGCCACCTCCACCAAGCGCCAATCAGATTGGACTGCCTAGCGCTCAGGTGGCCGCAGCCGAACGAAGAAAGAGGGAAGAACACCAGCGGTGGTACGAGAAAGAAAAGGCCCGGCTGGAAGAGGAACGCGAGAGAAAGCGCAGAGAGCAAGAGCGGAAACTGGGCCAGATGCGGACCCAGTCTCTGAACCCAGCTCCTTTCAGCCCTCTGACAGCCCAGCAGATGAAGCCTGAGAAGCCCTCCACACTGCAAAGACCCCAAGAGACAGTGATCCGCGAGCTGCAGCCACAGCAGCAACCTAGAACCATCGAGCGGAGGGACCTGCAGTACATTACCGTGTCCAAAGAAGAACTGAGCAGCGGCGACTCTCTGAGCCCTGATCCTTGGAAGCGGGACGCCAAAGAAAAGCTCGAGAAGCAGCAGCAGATGCACATCGTGGACATGCTGTCTAAAGAGATCCAAGAACTGCAGAGCAAGCCTGACAGAAGCGCCGAGGAAAGCGACAGACTGCGGAAGCTGATGCTCGAGTGGCAGTTCCAGAAGAGACTGCAAGAGTCCAAGCAGAAGGACGAGGACGACGAGGAAGAAGAGGACGACGACGTCGACACCATGCTGATCATGCAGAGACTCGAGGCTGAGCGGAGAGCCAGACTCCAAGACGAAGAACGTCGCAGACAGCAGCAGCTTGAGGAAATGCGCAAGAGAGAGGCCGAGGACCGGGCCAGACAAGAGGAAGAAAGACGGCGGCAAGAAGAAGAGAGGACCAAGAGGGACGCCGAAGAGAAGCGCCGACAAGAAGAGGGCTACTACAGCAGGCTGGAAGCCGAGCGTAGAAGGCAGCATGATGAGGCCGCTCGGAGACTGCTGGAACCTGAAGCACCAGGACTGTGCAGACCTCCTCTGCCTAGAGACTACGAGCCACCTAGTCCTTCTCCAGCACCTGGCGCACCACCACCACCTCCACAGAGAAATGCCAGCTACCTGAAAACTCAGGTGCTGAGCCCAGATAGCCTGTTCACCGCCAAGTTTGTGGCCTACAACGAGGAAGAGGAAGAAGAAGATTGCAGCCTGGCTGGCCCCAACAGCTATCCTGGATCTACTGGTGCTGCCGTGGGAGCCCACGATGCTTGCAGAGATGCTAAAGAGAAACGGTCCAAGAGCCAGGACGCCGACTCTCCAGGATCTTCTGGCGCCCCAGAGAACCTGACCTTTAAAGAGCGGCAGAGACTGTTCTCCCAGGGCCAAGACGTGTCCAACAAAGTGAAGGCCTCTAGGAAGCTGACCGAGCTGGAAAACGAGCTGAATACCAAGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSDYKDDDDKDYKDDDDKGSMSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDV
IETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKK
REKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTT
DPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERAD
GSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETY
VEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGM
VKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMV
SMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMS
LLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCF
KLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHT
RSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQ
ERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHR
SSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQR
VTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPP
PVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQ
RPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQ
ESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEA
ARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKR
SKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK*
IETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKK
REKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTT
DPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERAD
GSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETY
VEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGM
VKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMV
SMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMS
LLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCF
KLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHT
RSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQ
ERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHR
SSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQR
VTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPP
PVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQ
RPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQ
ESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEA
ARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKR
SKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK*
Vector Type
Mammalian
Antibiotic
Amp
Full Snapgene Map
pcDNA5 FRT/TO 3FLAG Afadin (MLLT4)96.57 KBSnapgene files can be opened using software available here: www.snapgene.com
DU Number
DU58266
Unit Source
Location
PPU
MTA
Price
£125.00
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