Expresses ABIN1 Nter KI antisense guide RNA, 20mer target sequence shown as sequence of insert with remaining 3 bases of PAM site in brackets (extra G added to improve expression from U6 promoter). Targets close to the ATG start codon in exon 2, PAM site situated on antisense strand. Designed to introduce a GFP tag to Abin1 via CRISPR when used in conjunction with the sense guide DU48486 and the donor plasmid DU48524. To be screened for using GFP followed by downstream PCR and Western analysis.
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