Plasmid
pcDNA5-FRT/TO-FLAG mouse STIM1 (with mutation)
Parent Plasmid
pcDNA5 FRT/TO FLAG
Expressed
FLAG mouse STIM1
Genbank
NM_009287.4 with mutation a2014g (K672E)
Tag
FLAG
Species
Mouse
Sequence of Insert
View Sequence
ggatccatggatgtgtgcgcccgtcttgccctgtggcttctttgggggct
ccttctgcatcagggccagagtctcagccatagtcacagtgaaaagaata
caggagctagctccggggcgacttctgaagagtctaccgaagcagagttt
tgccgaattgacaagcccctgtgccacagtgaggatgagaagctcagctt
tgaggccgtccgaaacatccataagctgatggatgacgatgccaatggtg
atgtggatgtggaagaaagtgatgagttcctaagggaagacctcaattac
catgacccaacagtgaaacatagcaccttccatggtgaggataagcttat
cagcgtggaggacctgtggaaggcgtggaagtcatcagaagtgtacaact
ggactgtggatgaggtgatacagtggctcattacgtatgtggagctgcca
cagtatgaggaaaccttccggaagttgcagcttactggccacgccatgcc
aaggctagcagtaaccaacaccaccatgacagggactgtactgaagatga
cagatcggagccacaggcagaagctgcagctgaaggccctggacacagtg
ctgtttgggcctcctctcttgactcggcataatcacctgaaggacttcat
gctggtggtgtctatcgttattggtgtgggtggctgctggtttgcctata
tccagaaccgttactctaaggagcacatgaagaaaatgatgaaggatctg
gaagggttacaccgggctgagcagagtctgcatgaccttcaggaaaggct
gcacaaggcccaggaggagcaccgaactgtggaagtagagaaggtccacc
tggagaagaagctgcgagatgagatcaaccttgccaagcaggaagctcag
cggctgaaggagctgagggagggtactgagaatgagaggagccgtcaaaa
atatgctgaggaagagctggagcaggttcgggaggccttgaggaaagcag
agaaggagctggaatcacacagctcatggtatgctcctgaggccctgcag
aagtggctgcagctgacccatgaggtggaggtgcagtactacaacatcaa
gaagcaaaatgcagagaggcagctgctggtggccaaggagggggctgaga
aaataaaaaagaagagaaacacgctttttggtaccttccatgtggcccac
agctcttccctggatgatgtggatcataaaatcctaactgctaagcaagc
tctgagtgaggtgacagcggcactgagggagcgcctgcaccggtggcagc
agatcgagatcctctgcggtttccagattgtcaataaccccggcatccac
tccttggtggctgctctcaacatcgaccccagctggatgggcagcacccg
ccctaaccccgcccacttcatcatgactgacgatgtggatgacatggatg
aggagattgtgtcgcccttgtccatgcagtcccccagcctgcagagcagt
gtccggcagcgcctgacggagccacagcttggcctgggatctcagaggga
tttgacccattccgattcggagtcctccctccacatgagtgaccgccagc
gtgtggcccccaagcctcctcagatgggccgtgctgcagatgaagctctc
aatgccatgccttccaatggcagccatcggctgattgagggggtccatcc
aggatctctggtggagaaactgcctgacagccctgctctggccaagaaga
catttatggcgttgaaccatggcctagacaaggcccacagcctgatggag
ctgaacccctcagtcccacctggtggctccccacttttggattcttccca
ttctcttagccccagttccccagacccagacacgccatctccagttgggg
acaaccgagctctgcagggtagccgaaacacacgaattccccacttggct
ggcaagaaggcaatggctgaggaggataatggttccattggtgaggagac
agactccagtccaggcagggagaagtttcctctcaaaatttttaagaagc
ctcttaagaagtaggcggccgc
ccttctgcatcagggccagagtctcagccatagtcacagtgaaaagaata
caggagctagctccggggcgacttctgaagagtctaccgaagcagagttt
tgccgaattgacaagcccctgtgccacagtgaggatgagaagctcagctt
tgaggccgtccgaaacatccataagctgatggatgacgatgccaatggtg
atgtggatgtggaagaaagtgatgagttcctaagggaagacctcaattac
catgacccaacagtgaaacatagcaccttccatggtgaggataagcttat
cagcgtggaggacctgtggaaggcgtggaagtcatcagaagtgtacaact
ggactgtggatgaggtgatacagtggctcattacgtatgtggagctgcca
cagtatgaggaaaccttccggaagttgcagcttactggccacgccatgcc
aaggctagcagtaaccaacaccaccatgacagggactgtactgaagatga
cagatcggagccacaggcagaagctgcagctgaaggccctggacacagtg
ctgtttgggcctcctctcttgactcggcataatcacctgaaggacttcat
gctggtggtgtctatcgttattggtgtgggtggctgctggtttgcctata
tccagaaccgttactctaaggagcacatgaagaaaatgatgaaggatctg
gaagggttacaccgggctgagcagagtctgcatgaccttcaggaaaggct
gcacaaggcccaggaggagcaccgaactgtggaagtagagaaggtccacc
tggagaagaagctgcgagatgagatcaaccttgccaagcaggaagctcag
cggctgaaggagctgagggagggtactgagaatgagaggagccgtcaaaa
atatgctgaggaagagctggagcaggttcgggaggccttgaggaaagcag
agaaggagctggaatcacacagctcatggtatgctcctgaggccctgcag
aagtggctgcagctgacccatgaggtggaggtgcagtactacaacatcaa
gaagcaaaatgcagagaggcagctgctggtggccaaggagggggctgaga
aaataaaaaagaagagaaacacgctttttggtaccttccatgtggcccac
agctcttccctggatgatgtggatcataaaatcctaactgctaagcaagc
tctgagtgaggtgacagcggcactgagggagcgcctgcaccggtggcagc
agatcgagatcctctgcggtttccagattgtcaataaccccggcatccac
tccttggtggctgctctcaacatcgaccccagctggatgggcagcacccg
ccctaaccccgcccacttcatcatgactgacgatgtggatgacatggatg
aggagattgtgtcgcccttgtccatgcagtcccccagcctgcagagcagt
gtccggcagcgcctgacggagccacagcttggcctgggatctcagaggga
tttgacccattccgattcggagtcctccctccacatgagtgaccgccagc
gtgtggcccccaagcctcctcagatgggccgtgctgcagatgaagctctc
aatgccatgccttccaatggcagccatcggctgattgagggggtccatcc
aggatctctggtggagaaactgcctgacagccctgctctggccaagaaga
catttatggcgttgaaccatggcctagacaaggcccacagcctgatggag
ctgaacccctcagtcccacctggtggctccccacttttggattcttccca
ttctcttagccccagttccccagacccagacacgccatctccagttgggg
acaaccgagctctgcagggtagccgaaacacacgaattccccacttggct
ggcaagaaggcaatggctgaggaggataatggttccattggtgaggagac
agactccagtccaggcagggagaagtttcctctcaaaatttttaagaagc
ctcttaagaagtaggcggccgc
Amino Acid Sequence
View Sequence
MDYKDDDDKGSMDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGAT
SEESTEAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESD
EFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQ
WLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQK
LQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKE
HMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDE
INLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHS
SWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNT
LFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGF
QIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLS
MQSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQ
MGRAADEALNAMPSNGSHRLIEGVHPGSLVEKLPDSPALAKKTFMALNHG
LDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPVGDNRALQGS
RNTRIPHLAGKKAMAEEDNGSIGEETDSSPGREKFPLKIFKKPLKK*
SEESTEAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESD
EFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQ
WLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQK
LQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKE
HMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDE
INLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHS
SWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNT
LFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGF
QIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLS
MQSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQ
MGRAADEALNAMPSNGSHRLIEGVHPGSLVEKLPDSPALAKKTFMALNHG
LDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPVGDNRALQGS
RNTRIPHLAGKKAMAEEDNGSIGEETDSSPGREKFPLKIFKKPLKK*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU16406
Unit Source
Location
PPU
MTA
Price
£125.00
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