Plasmid
pETDuet 6His-SLX4/Rad1
Parent Plasmid
pETDuet1
Genbank
Z73307 (SLX4) M15435.1 GI:172324 (Rad1)
Tag
6His
Species
Yeast
Sequence of Insert
View Sequence
DNA sequence of SLX4 insert :
ggatccgGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACA
AAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATA
AAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTA
GGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAG
CCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAA
AATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAAT
GCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAA
ACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATA
TAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCA
CCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACG
CGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATG
ACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGA
GATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACC
GGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTA
TAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTT
GAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATAT
TTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAG
ACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCC
ACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTT
GGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGT
TAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAA
GCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCC
TCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACA
TAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTA
AACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTC
TGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCC
AATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCT
ACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTC
TCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTC
GACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAA
ATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCC
AGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAG
CGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCT
GTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAG
AATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGggatcc
DNA sequence of Rad1 insert :
catATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCAGGA
GGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTAAAA
ATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAATGAA
GATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACCTAA
TGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTAGGC
CAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTGGTA
GAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGGACT
AGGATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACACCAA
CATCCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAATGCA
AAACCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTCGTG
GTTCTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCGATG
ATGAACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTGAGC
ATTGAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCATTAC
TTCTAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAAATA
GGGTTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAATTCG
AATGAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGGTTT
TATTAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTTCAC
CCCTCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTATGG
CCGAGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAAGAC
GTCACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCATGT
CTCAGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCTGAG
TTAAGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGAAAA
TGTCCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGCCGA
ACTGGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATAAGA
TTCCTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGACTT
TTTTGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTATCCC
GAAAATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTAGTC
ATATCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTTAGA
AGAAAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTTCAC
ATGAGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGTTCC
GACAACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAACAA
AAAAAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACAGAA
AACAGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAGGAC
ACTTTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAAAGA
ACAAGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTGCGG
CCGTTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTGGTT
TTTGAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGATGA
TTTGGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTTTTG
AAATACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCTGAA
TTTGACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACATCAC
AACCCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATGAAT
ATGTTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTCAAT
GACAATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTTTGA
ACCAGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAGTGA
AGGATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATTGAA
GAGCAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCACAAA
GTTGATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATGAAG
ATCTTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCAAAA
TTACGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCATAA
TCTTACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCTCAT
TACCAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATGTTG
ACAGTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAAATC
GATTTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACCAAT
GTAAAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAGTTT
GATGAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAATTA
TAAGAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTATCCC
AGGATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCCACT
TTAAAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAATCCT
AGAGTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTATAT
TGGGAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGCCTG
AAGGATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCCTGG
AGTGTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCTTCA
ATAAGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAATGAC
GAAGATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGAAGA
ACAAGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGACCA
CTGATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCACCT
GTGTAActcgag
ggatccgGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTACCGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGATTCAATAGGTACGCAAGTAAAAAGCGTAACTGCACA
AAAATCGCCTATGACGCAAGAGACTACGAAAAACGATACAGAAAGAAATA
AAGATGTTGATAAAAGTTGTAACCCAGTCAGCACTTCCCATCCCGATTTA
GGCGGATCAAATATCGAGGAGAACATATTCATTAACACTCAAATTCAAAG
CCGACTTGATGACGCAGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAA
AATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAAT
GCAAATGTAACAGCTAAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAA
ACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATA
TAACTGATTTCAATATTAATAACTACGAACGTTCAAGGACTGCCAGCTTA
TTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGAC
GCAAAATGAAGGAAACAGTGATAAACCTCCCAGAGCAAGGAATAACAAGG
GTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATT
ATGAAACTTCTTCTACAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAA
TGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAG
ACAATCAAGAATCCTCACAACAAAGACTTTGGACAGCATCACAATTACCA
CCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACG
CGATACCCAAAGTGCAGTAAATTTCTTATCGTTGTCTCAAGTAATGGATG
ACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGA
GATTCTACAAGCTCTCAAGAATATGGAAACGGGTTAGAGCCACAACAACC
GGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTA
TAAATGCATTTTCACTGACAGATTATAAAGCTTGCAAACCTATGTCTGTT
GAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATAT
TTCTATTGTCTCAGATACTACAGATGAGACATCCACATTGTTTCCACTAG
ACCAGTATCGGTATGTTTTCATTGAAAATGATGAAAGACCACCATTAGCC
ACCGACACGATAGGCAGCACACAGTTTTTCACTCCAAACACATCGCCTTT
GGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGT
TAATATCACCTTTGAAAGTAGAAAACAACAAAACAGGGGTAACATCACAA
GCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCC
TCAAAAGAACCTTACTACAACTTTAAAAACTGAAGAAGAAAAGAATAACA
TAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTA
AACCCCAAACTGGTGAAACATAACTTTTATAGAGTTGAGGCAAATGATTC
TGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCC
AATTGGTAGATTCCTCCAAAATTTCGACTAAAGATTCTACACAAAATCCT
ACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTC
TCCCGAGAAATTTTGTGAGATCATGATGTCCCAATCAATGAAAGAGCTTC
GACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAA
ATCATTCAATCCTTACAAACTGCATCTCAAATTCTGTCCACAGCTAATCC
AGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAA
TATTTGATCATTTAACCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAG
CGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCT
GTTCAGCGCCGAGCCATTTGTTTCGCAAATCGACGAAATGACCATCAGAG
AATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGggatcc
DNA sequence of Rad1 insert :
catATGTCTCAGTTATTTTATCAGGGCGACTCTGATGATGAGCTCCAGGA
GGAACTTACGAGGCAGACAACTCAAGCATCTCAAAGTTCTAAAATTAAAA
ATGAAGATGAACCCGACGACTCCAATCATCTTAATGAGGTGGAAAATGAA
GATAGCAAAGTTTTAGATGACGATGCAGTGTTATACCCTCTTATACCTAA
TGAGCCAGATGACATAGAAACGTCTAAGCCCAATATTAACGATATTAGGC
CAGTTGATATTCAATTGACTTTACCATTGCCGTTTCAGCAAAAAGTGGTA
GAGAATTCATTAATTACTGAAGATGCATTAATCATAATGGGGAAAGGACT
AGGATTGCTTGATATTGTGGCCAATTTATTGCATGTTTTAGCTACACCAA
CATCCATTAACGGACAACTAAAGCGAGCGCTCGTCCTAGTGTTGAATGCA
AAACCTATAGATAATGTAAGAATCAAGGAGGCCTTAGAAGAGCTGTCGTG
GTTCTCTAATACTGGGAAGGACGACGACGATACTGCTGTCGAGAGCGATG
ATGAACTTTTTGAAAGGCCTTTTAACGTAGTTACCGCGGACTCGCTGAGC
ATTGAAAAGAGAAGAAAGCTATATATTTCTGGCGGAATCTTGAGCATTAC
TTCTAGAATTCTCATTGTGGATCTCTTATCCGGCATTGTTCACCCAAATA
GGGTTACGGGTATGCTGGTATTGAATGCAGACTCACTTCGACATAATTCG
AATGAATCGTTTATATTAGAGATTTACAGGTCTAAAAATACTTGGGGTTT
TATTAAAGCCTTTTCTGAAGCACCAGAGACGTTTGTCATGGAATTTTCAC
CCCTCAGGACGAAAATGAAAGAATTACGGCTAAAGAACGTTTTGCTATGG
CCGAGGTTCAGGGTAGAGGTCTCTTCCTGTTTGAATGCCACTAATAAGAC
GTCACACAATAAAGTCATTGAAGTCAAGGTCTCCTTAACAAATTCCATGT
CTCAGATACAGTTTGGCTTGATGGAATGTTTGAAAAAATGTATTGCTGAG
TTAAGCAGAAAAAATCCTGAACTAGCTCTGGACTGGTGGAATATGGAAAA
TGTCCTGGATATAAACTTTATCAGGTCAATTGACTCGGTGATGGTGCCGA
ACTGGCACCGAATTTCTTATGAATCAAAACAACTGGTTAAGGATATAAGA
TTCCTACGCCACCTTTTAAAGATGCTCGTAACTTCAGACGCAGTTGACTT
TTTTGGAGAGATTCAATTAAGTTTGGATGCCAATAAACCGTCAGTATCCC
GAAAATACAGCGAATCACCGTGGCTATTGGTCGATGAGGCACAATTAGTC
ATATCGTATGCGAAGAAAAGAATATTTTACAAAAATGAATATACTTTAGA
AGAAAATCCAAAATGGGAACAACTTATTCATATATTACATGATATTTCAC
ATGAGAGAATGACCAATCACCTTCAGGGGCCTACTTTAGTTGCCTGTTCC
GACAACCTTACATGTTTAGAACTAGCAAAGGTCTTGAATGCCTCAAACAA
AAAAAGAGGAGTACGTCAAGTGCTTCTGAATAAATTGAAATGGTACAGAA
AACAGAGGGAGGAAACGAAAAAATTGGTCAAAGAAGTGCAAAGTCAGGAC
ACTTTTCCAGAGAATGCAACATTAAATGTAAGCTCGACATTTTCCAAAGA
ACAAGTGACCACGAAAAGAAGAAGGACAAGAGGTGCTTCACAAGTTGCGG
CCGTTGAAAAGCTAAGGAATGCAGGTACCAATGTAGATATGGAGGTGGTT
TTTGAGGATCATAAGTTATCTGAAGAAATTAAGAAGGGAAGCGGTGATGA
TTTGGATGACGGTCAGGAAGAAAATGCCGCAAACGATTCAAAGATTTTTG
AAATACAAGAACAGGAAAATGAAATCCTTATCGATGATGGGGATGCTGAA
TTTGACAACGGAGAATTAGAGTATGTGGGCGACCTTCCGCAGCACATCAC
AACCCATTTCAATAAGGATTTATGGGCAGAACATTGCAACGAGTATGAAT
ATGTTGATCGTCAGGACGAAATTTTAATCTCTACGTTTAAAAGTCTCAAT
GACAATTGCTCATTGCAGGAGATGATGCCCTCTTACATTATAATGTTTGA
ACCAGATATATCGTTTATCAGGCAGATTGAAGTTTATAAGGCCATAGTGA
AGGATTTGCAACCAAAAGTATACTTCATGTACTACGGTGAAAGTATTGAA
GAGCAAAGTCATTTGACTGCTATCAAGAGAGAGAAAGATGCTTTCACAAA
GTTGATTAGAGAGAATGCAAATCTGTCCCATCACTTTGAAACGAATGAAG
ATCTTTCTCACTACAAAAATTTAGCTGAAAGGAAGTTGAAGCTTTCAAAA
TTACGAAAATCTAATACCAGAAATGCGGGTGGGCAGCAGGGATTCCATAA
TCTTACTCAGGATGTCGTCATTGTGGATACACGTGAGTTTAATGCCTCAT
TACCAGGCTTACTCTACCGATATGGCATAAGGGTTATTCCTTGTATGTTG
ACAGTCGGCGATTATGTGATAACTCCTGATATTTGTCTCGAAAGAAAATC
GATTTCTGACTTAATTGGGTCATTACAGAATAACAGATTAGCCAACCAAT
GTAAAAAAATGTTAAAATACTATGCATATCCGACACTATTGATTGAGTTT
GATGAAGGACAGTCGTTTTCTTTAGAACCTTTTAGTGAACGTAGAAATTA
TAAGAATAAAGACATATCAACTGTTCATCCTATATCAAGCAAGTTATCCC
AGGATGAAATTCAGCTAAAACTAGCCAAATTAGTATTGCGGTTTCCCACT
TTAAAGATTATATGGTCTTCCTCACCCCTGCAAACTGTAAATATAATCCT
AGAGTTGAAATTAGGACGTGAGCAACCTGACCCTAGTAATGCAGTTATAT
TGGGAACGAATAAAGTTAGATCGGATTTTAATAGCACTGCAAAGGGCCTG
AAGGATGGTGATAACGAGTCTAAATTCAAGAGACTGTTGAATGTTCCTGG
AGTGTCAAAAATTGATTATTTCAATCTCCGCAAAAAGATCAAGAGCTTCA
ATAAGCTTCAAAAGCTTTCATGGAATGAGATTAATGAACTTATTAATGAC
GAAGATTTGACGGATAGAATATACTACTTCTTGAGAACAGAAAAAGAAGA
ACAAGAACAAGAGTCAACAGATGAAAATCTTGAATCTCCTGGTAAGACCA
CTGATGATAACGCTTTACATGATCATCATAATGATGTTCCTGAAGCACCT
GTGTAActcgag
Amino Acid Sequence
View Sequence
Amino acid sequence of expressed 6His-SLX4 protein :
MGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQN
PTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*
Amino acid sequence of expressed 6His-SLX4 protein.
MGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQN
PTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*
Amino acid sequence of Rad1 ;
MSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDEPDDSNHLNEVENED
SKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDIQLTLPLPFQQKVVE
NSLITEDALIIMGKGLGLLDIVANLLHVLATPTSINGQLKRALVLVLNAK
PIDNVRIKEALEELSWFSNTGKDDDDTAVESDDELFERPFNVVTADSLSI
EKRRKLYISGGILSITSRILIVDLLSGIVHPNRVTGMLVLNADSLRHNSN
ESFILEIYRSKNTWGFIKAFSEAPETFVMEFSPLRTKMKELRLKNVLLWP
RFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQIQFGLMECLKKCIAEL
SRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHRISYESKQLVKDIRF
LRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYSESPWLLVDEAQLVI
SYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERMTNHLQGPTLVACSD
NLTCLELAKVLNASNKKRGVRQVLLNKLKWYRKQREETKKLVKEVQSQDT
FPENATLNVSSTFSKEQVTTKRRRTRGASQVAAVEKLRNAGTNVDMEVVF
EDHKLSEEIKKGSGDDLDDGQEENAANDSKIFEIQEQENEILIDDGDAEF
DNGELEYVGDLPQHITTHFNKDLWAEHCNEYEYVDRQDEILISTFKSLND
NCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQPKVYFMYYGESIEE
QSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSHYKNLAERKLKLSKL
RKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGLLYRYGIRVIPCMLT
VGDYVITPDICLERKSISDLIGSLQNNRLANQCKKMLKYYAYPTLLIEFD
EGQSFSLEPFSERRNYKNKDISTVHPISSKLSQDEIQLKLAKLVLRFPTL
KIIWSSSPLQTVNIILELKLGREQPDPSNAVILGTNKVRSDFNSTAKGLK
DGDNESKFKRLLNVPGVSKIDYFNLRKKIKSFNKLQKLSWNEINELINDE
DLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDNALHDHHNDVPEAPV
*
MGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQN
PTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*
Amino acid sequence of expressed 6His-SLX4 protein.
MGSSHHHHHHSQDPELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDDKSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQN
PTTSNDIIDTSAASSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*
Amino acid sequence of Rad1 ;
MSQLFYQGDSDDELQEELTRQTTQASQSSKIKNEDEPDDSNHLNEVENED
SKVLDDDAVLYPLIPNEPDDIETSKPNINDIRPVDIQLTLPLPFQQKVVE
NSLITEDALIIMGKGLGLLDIVANLLHVLATPTSINGQLKRALVLVLNAK
PIDNVRIKEALEELSWFSNTGKDDDDTAVESDDELFERPFNVVTADSLSI
EKRRKLYISGGILSITSRILIVDLLSGIVHPNRVTGMLVLNADSLRHNSN
ESFILEIYRSKNTWGFIKAFSEAPETFVMEFSPLRTKMKELRLKNVLLWP
RFRVEVSSCLNATNKTSHNKVIEVKVSLTNSMSQIQFGLMECLKKCIAEL
SRKNPELALDWWNMENVLDINFIRSIDSVMVPNWHRISYESKQLVKDIRF
LRHLLKMLVTSDAVDFFGEIQLSLDANKPSVSRKYSESPWLLVDEAQLVI
SYAKKRIFYKNEYTLEENPKWEQLIHILHDISHERMTNHLQGPTLVACSD
NLTCLELAKVLNASNKKRGVRQVLLNKLKWYRKQREETKKLVKEVQSQDT
FPENATLNVSSTFSKEQVTTKRRRTRGASQVAAVEKLRNAGTNVDMEVVF
EDHKLSEEIKKGSGDDLDDGQEENAANDSKIFEIQEQENEILIDDGDAEF
DNGELEYVGDLPQHITTHFNKDLWAEHCNEYEYVDRQDEILISTFKSLND
NCSLQEMMPSYIIMFEPDISFIRQIEVYKAIVKDLQPKVYFMYYGESIEE
QSHLTAIKREKDAFTKLIRENANLSHHFETNEDLSHYKNLAERKLKLSKL
RKSNTRNAGGQQGFHNLTQDVVIVDTREFNASLPGLLYRYGIRVIPCMLT
VGDYVITPDICLERKSISDLIGSLQNNRLANQCKKMLKYYAYPTLLIEFD
EGQSFSLEPFSERRNYKNKDISTVHPISSKLSQDEIQLKLAKLVLRFPTL
KIIWSSSPLQTVNIILELKLGREQPDPSNAVILGTNKVRSDFNSTAKGLK
DGDNESKFKRLLNVPGVSKIDYFNLRKKIKSFNKLQKLSWNEINELINDE
DLTDRIYYFLRTEKEEQEQESTDENLESPGKTTDDNALHDHHNDVPEAPV
*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU11451
Unit Source
Location
PPU
MTA
Price
£125.00
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