SLX41P was immunoprecipitated from HEK293 human cells (2 mg / IP) using the 2nd bleed anti-SLX41P S587D (05 or 2 ug). As a negative control, pre-immune IgG was used. Anti-SLX41P S587D was used for immunoblotting for 1 hour at 1 ug/ml or 0.2 ug/ml overnight in 5 % milk TBS-tween. 5, 15 and 40 ugs were loaded in the same gel and anti-SLX41P S587D recognises a single band compatible with SLX41P in lysates.

SLX41P was immunoprecipitated from MEF mouse cells (2 mg / IP) using the 2nd bleed anti-SLX41P S587D (05 or 2 ug). As a negative control, pre-immune IgG was used. Anti-SLX41P S587D was used for immunoblotting for 1 hour at 1 ug/ml or 0.2 ug/ml overnight in 5 % milk TBS-tween. 5, 15 and 40 ugs were loaded in the same gel and anti-SLX41P S587D recognises a single band compatible with SLX41P in lysates. In immunoprecipitations, mouse SLX41P migrates at the same size as the IgG light chain.