Polyclonal Antibody - IRF5

Name: IRF5
Price per aliquot (100µg): £110.00
Full Name:
IRF5 phospho Ser 462
Long Name Text:
interferon regulatory factor 5
Immuno Sequence:
IRLQIS*NPDLK [residues 457 - 467 of human]
Phosphopeptide:
IRLQIS*NPDLK [residues 457 - 467 of human]
Aliases:
IRF5
Sheep Number:
S509D
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

HEK293T cells were co-transfected for 48 hours with FLAG-IRAK1 and HA-IRF5 wildtype and HA-IRF5 S362A. 20 ug of cell lysate protein were subjected to SDS-Page and immunoblotted overnight with 1 ug/ml of anti-IRF5 phospho Ser 462 plus 20 ug/ml of the unphosphorylated form of the peptide immunogen or for one hour with anti-HA (Sigma-Aldrich).
Notes:
1 ug/ml for immunoblotting with 20 ug/ml of non-phospho peptide.

Do you need any help? Please get in touch and we’ll be happy to lend a hand.