Polyclonal Antibody - IRF5

Name: IRF5
Price per aliquot (100µg): £110.00
Full Name:
Long Name Text:
interferon regulatory factor 5
Immuno Sequence:
GST-IRF5 [DU 35469]
DU Number of Antigen:
Sheep Number:
Tag Information:
Tagged with GST.
Purification Method:
Affinity purified against recombinant protein GST-IRF5 [DU 35469] and the depleted against GST.
Gel Image:

A. 0.5 mg of Gen2.2 cell lysate protein were immunoprecipitated with the indicated concentration of IRF5 antibody (S485D) and for the times indicated. The immunoprecipitates were subjected to SDS-PAGE and immunoblotted with an IRF5 antibody from Cell Signaling Technology. B. Gen2.2 cells were either left unstimulated or stimulated with the TLR7 agonist CL097 (1 μg/ml) for 30 min. 1 mg of cell lysate protein was incubated overnight with 2 μg/μl of IRF5 antibody (S485D, third bleed) or pre-immune IgG as a control. The immunoprecipitates and the supernatants were subjected to SDS-PAGE and immunoblotted with IRF5 antibody (Cell Signaling Technology). The results show that IRF5 can be immune-depleted almost completely from the cell lysates.
0.5 - 2 ug of antibody per 0.5 mg of cell extract for immunoprecipitation

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