Polyclonal Antibody - UBXD7

Name: UBXD7
Price per aliquot (100µg): £110.00
Full Name:
Long Name Text:
UBX domain protein 7
Immuno Sequence:
GST-UBXD7 [DU 23280]
DU Number of Antigen:
Sheep Number:
Tag Information:
Tagged with GST.
Purification Method:
Affinity purified against recombinant protein and then depleted against GST.
Gel Image:

Western Blot with HeLa cell extracts [25 μg/lane] from UBXD7 WT expressing or UBXD7 depleted or over-expressing HeLa cells. Total cell extracts were immunoblotted with purified anti-UBXD7 antibodies (5% milk in TBS-Tween, o/n). Binding of the primary antibody was detected using peroxidase conjugated anti-sheep IgG antibody (1:3000 dilution) followed by enhanced chemiluminescence (ECL, Amersham).
Mouse brain lysate (9.4 mg protein lysate) was immunoprecipitated with 80 μl anti- UBXD7 crosslinked to Protein A (PrA) agarose beads (dry beads). For the antibody crosslinking: 250 μg anti-UBXD7 antibody were crosslinked to 1 ml Protein A agarose beads [dry beads]. WB: load 50 μg for Input, 8% of the IP

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