Polyclonal Antibody - SIK2

Name: SIK2
Price per aliquot (100µg): £110.00
Full Name:
SIK2 phospho Thr 175
Long Name Text:
salt-inducible kinase 2
Immuno Sequence:
GELLAT*WCGSP [residues 170 - 180 of human]
GELLATWCGSP [residues 170 - 180 of human]
Sheep Number:
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

HEK293 cells were transfected with vectors encoding HA-SIK1, HA-SIK2 or HA-SIK3 wildtype (WT) or carrying an alanine mutation at the T-loop site. Cell extracts were then blotted using the anti-SIK1 phospho Thr 175 antibody S405D. Detected very strongly SIK1, SIk2 and SIK3 wildytype in extracts of HEK293 cells overexpressing wildtype HA-SIK1, HA-SIK2 or HA-SIK3, but not the protein carrying a mutation at the T-loop site.
Please note: This antibody has not tested on endogenous protein. This antibody cross- reacts with SIK2 and SIK3 and may cross-react with other members of the AMPK family. One should therefore immunoprecipitate the relevant SIK isoform prior to immunoblott

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