Polyclonal Antibody - TRAFD1

Name: TRAFD1
Price per aliquot (100µg): £110.00
Full Name:
GST-TRAFD1 (101 - 582)
Long Name Text:
TRAF type zinc finger domain containing 1
Immuno Sequence:
GST-TRAFD1 (101 - 582) [SC 20511]
Sheep Number:
Tag Information:
Tagged with GST.
Purification Method:
Affinity purified against GST-TRAFD1 (101 – 582) [DU 20511] and then depleted against GST.
Gel Image:

RAW264.7 cells were treated with IFNβ for the indicated times (to induce expression of TRAFD1) and with the indicated concentrations. 25 μg total cell lysate was loaded on a 10 % polyacrylamide gel and blotted overnight at 4 °C with anti-TRAFD1 in 5 % milk at the indicated concentrations. Secondary HRP conjugated anti-Sheep antibody was used at 1:10,000 dilution for 1 h at room temperature. Bleeds 1, 2 and 3 performed similarly. The above image is from bleed 1.
RAW264.7 cells were stimulated with IFNβ for 8 h or left unstimulated as indicated. Cells were lysed and total cell lysate (lanes 1 and 2) was run alongside supernatants after immunoprecipitation of 1 mg cell lysate with 0.5, 1, or 2 μg anti-TRAFD1 (lanes 3-8) or 2 μg of sheep pre-immune IgG (lanes 9 and 10) coupled to ProteinG-Sepharose beads. 25 μg of lysate was run in each case. The above image is from bleed 1.

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