Polyclonal Antibody - Sharpin

Name: Sharpin
Price per aliquot (100µg): £110.00
Full Name:
GST-Sharpin
Long Name Text:
SHANK-Associated RH Domain Interactor
Immuno Sequence:
GST-Sharpin [DU 32976]
DU Number of Antigen:
DU32978
Sheep Number:
S209D
Tag Information:
Tagged with GST.
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against recombinant protein GST- SHARPIN [DU 32976] and then depleted against GST.
Gel Image:

HEK293-ILIR cells were incubated without or with IL-Ibeta and SHARPIN was precipitated from 3 mg cell lysates using immunobilised NEMP (IKK gamma), which captures linear and Lys63-linked ubiquitin chains. Eluted proteins were subjected to SDS-Page before being immunoblotted with anti-SHARPIN. The results show that NEMO captures SHARPIN only in IL1-stimulated cells. SHARPIN was not captured, when NEMO was replaced by the polyubiquitin-binding defective mutants NEMO D311N.
SHARPIN was immunoprecipitated from HeLa total cell extracts (1 mg) using various amounts of anti-SHARPIN S209D before being immunoblotted with anti-HOIP (Proteintech 14626-1-AP).

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