COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Monoclonal Antibody - HA tag

Name: HA tag
Price per aliquot (100µg): £110.00
Full Name:
Long Name Text:
HA epitope tag
Immuno Sequence:
Sheep Number:
Mouse Monoclonal 12CA5
Gel Image:

HEK293 were transfected with a vector encoding HA-SIK2 and lysed 48 h post-transfection. Cell lysates (200 μg protein extract) were incubated with increasing amounts of the mouse monoclonal anti-HA antibody (12ca5) for 90 min at 4°C and then immune complexes were recovered by adding 10 μl of protein G- sepharose and incubating sample for a further 30 mins at 4°C. HA-SIK2 was also immunoprecipitated by incubating whole cell extracts with 5 μl of anti-HA coupled beads from Sigma. The immunoprecipitates were washed with lysis buffer and proteins bound to the column were eluted with 1% SDS, seperated by SDS-PAGE (8% gel) and immunoblotted using the rat monoclonal antibody (3F10) from Roche.

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