Polyclonal Antibody - IRF7

Name: IRF7
Price per aliquot (100µg): £110.00
Full Name:
GST-IRF7
Long Name Text:
interferon regulatory factor 7
Immuno Sequence:
GST-IRF7 [DU 12004]
DU Number of Antigen:
DU12004
Sheep Number:
S905C
Tag Information:
Tagged with GST.
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against recombinant protein and then depleted against GST.
Gel Image:

Lysates
 from
 Gen2.2,
 a
 plasmacytoid
 dendritic
 cell
 line
 (0.5
 mg
 protein),
 were
 incubated
 overnight
 with
 increasing
 amounts
 of
 anti‐IRF7
 antibody
 (3rd
 bleed)
 or
 pre‐immune
 serum
 (IgG).
 10
 μl
 of
 protein
 G
 were
 added
 for
 1
 extra
 hour
 to
 allow
 immunoprecipitation.
 Immunoprecipitated
 proteins
 were
 denatured
 in
 SDS
 and
 subjected
 to
 SDS‐PAGE.
 The
 IRF7
 protein
 in
 the
 immunoprecipitates
 (IP)
 or
 remaining
 in
 the
 supernatant
 (SN)
 was
 detected
 using
 a
 mouse
 monoclonal
 antibody
 against
 IRF7
 (clone
 G8;
 Santa
 Cruz,
 sc‐74472)
 overnight
 at
 1:1000.
 


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