Polyclonal Antibody - PINK

Name: PINK
Price per aliquot (100µg): £110.00
Full Name:
PINK (235 - end mouse)
Long Name Text:
PTEN induced putative kinase 1
Immuno Sequence:
PINK (235 - end mouse MBP cleaved) [DU 17570]
Phosphopeptide:
PINK (235 - end mouse MBP cleaved) [DU 17570]
DU Number of Antigen:
DU17570
Sheep Number:
S774C
Tag Information:
MBP tag cleaved off.
Monoclonal/Polyclonal:
Polyclonal
Gel Image:

HEK293 cells were either transfected with GFP empty vector/ GFP-PINK1 (125-end) mouse/ GFP-PINK1 (125-end) human and 25μg of protein was subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 (235-end) at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.
PINK1 was immunoprecipitated from 1mg HEK 293 cellular extracts over-expressing mouse 25 GFP-PINK1 (125-END). Immunoprecipitation were performed using 5ug of pre-immune serum covalently coupled to protein G sepharose (lane1) or 5ug anti-PINK1 S774C antibody covalently coupled to protein G sepharose (lane 2). Immunoprecipitates were subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 IB: HAX (DSTT A IB: OMI (DSTT Ab) S774C antibody at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.

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