Polyclonal Antibody - IRAK1

Name: IRAK1
Price per aliquot (100µg): £110.00
Full Name:
IRAK1 (mouse)
Long Name Text:
Interleukin-1 Receptor-Associated Kinase
Immuno Sequence:
GST cleaved IRAK1 mouse [DU 32043]
Phosphopeptide:
GST cleaved IRAK1 mouse [DU 32043]
DU Number of Antigen:
DU32043
Sheep Number:
S690C
Tag Information:
GST tag has been cleaved.
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified GST-IRAK1 (mouse) and the depleted against GST.
Gel Image:

Raw cell lysates (1mg protein) were immunoprecipitated with increasing amounts of mouse anti-IRAK1 antibody or pre-immune serum for 2hr along with 10 μl of protein G. Immunoprecipitated proteins were denatured in SDS and subjected to 8% SDS-PAGE (Lanes 1-4). Lanes 5- 8 indicate the amount of mouse IRAK1 protein remaining in the supernatant after immunoprecipitation, the amounts loaded being equivalent to 25 μg of extract protein. Proteins were transferred to PVDF membrane and immunoblotting was carried out using rabbit polyclonal antibody against IRAK1 (Santa Cruz) overnight at 1:1000.

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