Polyclonal Antibody - WNK1

Name: WNK1
Price per aliquot (100µg): £110.00
Full Name:
WNK1 (2360 - 2382)
Long Name Text:
WNK lysine deficient protein kinase 1
Immuno Sequence:
QNFNISNLQKSISNPPGSNLRTT [Residies 2360 - 2382 of human]
Phosphopeptide:
QNFNISNLQKSISNPPGSNLRTT [Residies 2360 - 2382 of human]
Aliases:
WNK1
Sheep Number:
S062B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against WNK1 (2360 – 2382) peptide.
Gel Image:

Rat tissue extracts (40 μg of protein) and HEK 293 cellular extract (40 μg of protein) were subjected to SDS-Page analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-WNK1 at 1 μg/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
WNK1 and pre immune IgG antibodies were covalently coupled to protein G Sepharose in a ratio of 1 mg of antibody to 1 ml of resin using a dimethyl pimelimidate cross- linking procedure. As a pre-clearing step, 50 mg of rat testis lysate was incubated at 4 °C for 20 mins on a rolling shaker with 0.5 ml of protein G-Sepharose. The supernatant was then incubated at 4 °C for 1 hour on a rolling shaker with 50 μl of WNK1 or IgG-Protein G-Sepharose conjugated antibodies. The immunoprecipitates were washed four times with 10 ml of 10 mM Tris-HCl pH 8 / 0.1 mM EGTA. The resin was resuspended in SDS-Page sample buffer and subjected to SDS-Page analysis with staining with Coomassie blue.

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