Polyclonal Antibody - TRAF6

Name: TRAF6
Price per aliquot (100µg): £110.00
Full Name:
TRAF6
Long Name Text:
TNF receptor-associated factor 6
Immuno Sequence:
GST-TRAF6 [DU 1758]
DU Number of Antigen:
DU1758
Aliases:
TRAF6
Sheep Number:
S529C
Tag Information:
Tagged with GST
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against recombinant protein MBP-TRAF6 [DU 15463]
Gel Image:

The indicated amounts of anti-TRAF6 antibodies or pre-immune control IgG were incubated for 16 h at 40C on a rotating wheel with 0.025 ml of cell extract (0.5 mg protein) from unstimulated IL-1R HEK293 cells and 7.5 μl of packed Protein G- Sepharose beads. After centrifugation to pellet the antibody-Sepharose conjugate, the supernatants were removed and the immunoprecipitates washed three times with 50 mM Tris-HCl pH 7.5, 1 % (v/v) Triton-X100, 0.05 % (v/v) 2-mercaptoethanol and 0.2 M NaCl. The supernatants and pellets were denatured in SDS and aliquots subjected to SDS-PAGE and immunoblotting using a commercial TRAF6 antibody from Santa Cruz (H-274).

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