Polyclonal Antibody - SPAK

Name: SPAK
Price per aliquot (100µg): £110.00
Full Name:
SPAK phospho Ser 373
Long Name Text:
Serine/threonine-protein kinase 39
Immuno Sequence:
RRVPGSS*GHLHKT [residues 367 - 379 of human]
Phosphopeptide:
RRVPGSSGHLHKT [residues 367 - 379 of human]
Sheep Number:
S670B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

HeLa cells were transfected with a control siRNA duplex or a siRNA duplex targeting human WNK1. 48 hours after transfection, cells were either left untreated or stimulated with 0.5 M sorbital for 30 mins. Total cell extracts (20 ug) were immunoblotted with the following antibodies: Anti-WNK1 (61 - 661) [S119B] Anti-SPAK phospho Thr 233 / OSR1 phospho Thr 185 [S688B] Anti-SPAK phospho Ser 373 / OSR1 phospho Ser 325 [S670B] Anti-SPAK / OSR1 [S637B + S636B] Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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