Polyclonal Antibody - SAP97

Name: SAP97
Price per aliquot (100µg): £110.00
Full Name:
SAP97
Long Name Text:
Sin3A-associated protein
Immuno Sequence:
GST-SAP97 [DU 391]
DU Number of Antigen:
DU391
Sheep Number:
S552B
Tag Information:
Tagged with GST.
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against recombinant GST-SAP97 and then depleted against GST
Gel Image:

Phosphorylation of SAP97/hDlg in HEK293 cells after cellular stress. Cells were incubated for 1 hour with or without 10 μM SB203580 and/or 5 μM PD184352, then exposed for 15 min to 0.5 M sorbitol or to UV C radiation (200J/m2), followed by a 30 min incubation. Endogenous SAP97/hDLg was immunoprecipitated from 1 – 5 mg of cellular lysate using 4 μg of anti-SAP97 (S552B), the resulting pellets were immunoblotted using antibodies that recognise SAP97 phosphorylated on serine 158 (anti-SAP97 phospho Ser 158 S285B), threonine 209 (anti-SAP97 phospho Thr 209 S938A), serine 431 (anti-SAP97 phospho Ser 431 S938A), serine 442 (anti-SAP97 phospho Ser 442 S965A) and an antibody that recognises unphosphorylated and phosphorylated SAP97 equally as well (anti-SAP97 S552B). The lanes in the panel are in duplicates. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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