Polyclonal Antibody - Ribosomal S6 protein

Name: Ribosomal S6 protein
Price per aliquot (100µg): £110.00
Full Name:
Ribosomal S6 protein phospho Ser 235
Long Name Text:
Ribosomal S6 protein
Immuno Sequence:
AKRRRKS*SLRASTS [residues 229 - 242 of human]
Phosphopeptide:
AKRRRKSSLRASTS [residues 229 - 242 of human]
Sheep Number:
S441A
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho-specific peptide.
Gel Image:

20 μg of unstimulated (U) and IGF stimulated (S) 293 cell lysates were separated by SDS-Page transferred to nitrocellulose membrane and blotted with the anti-ribosomal S6 phospho Ser 235 antibody at 1 μg/ml in the presence of 10 μg/ml of the non- phosphorylated peptide. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminscence (ECL, Amersham).

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