Polyclonal Antibody - PROTOR

Name: PROTOR
Price per aliquot (100µg): £110.00
Full Name:
PROTOR1
Long Name Text:
Protein observed with Rictor-1
Immuno Sequence:
GST-PROTOR1 (1 - 388) [DU 10413] [Accession number NM_181333] - Originally named PRR5
DU Number of Antigen:
DU10413
Aliases:
PRR5
Sheep Number:
S020C
Tag Information:
Tagged with GST.
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against recombinant protein and then depleted against GST.
Gel Image:

PROTOR1 was immunoprecipitated from 1 mg of HEK 293 and various mouse tissue clarified cellular extracts using 5 μg of anti-PROTOR1 antibody. The immunopecipitate was resolved by SDS-Page on a 4-12 % Bis-Tris gel and transferred to PVDF membrane. The membrane was then probed with anti-PROTOR1 antibodies at 1 μg/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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