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Polyclonal Antibody - PINK

Name: PINK
Price per aliquot (100µg): £110.00
Full Name:
PINK (125 - 539)
Long Name Text:
PTEN induced putative kinase 1
Immuno Sequence:
PINK (125 - 539) MBP cleaved [DU 13946]
DU Number of Antigen:
DU13946
Sheep Number:
S460C
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against MBP-PINK1 (125-end).
Gel Image:

HEK293 cells were either untransfected (lane one) or transfected with full length (1-581) PINK1-myc cDNA (lane two). 25μg of protein was subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 (125 – 539) at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.
PINK1 was immunoprecipitated from 1mg HEK 293 cellular extracts over-expressing humanwildtypePINK1. Immunoprecipitateswereperformedusing5μgofpre-immune serum covalently coupled to protein G sepharose (lane 1); 5μg anti-PINK1 S688B antibody covalently coupled to protein G sepharose (lane 2); 5μg anti-PINK1 S460C (2nd bleed) antibody covalently coupled to protein G sepharose (lane 3); 5μg anti-PINK1 S460C (3rd bleed) antibody covalently coupled to protein G sepharose (lane 4) or 5μg anti-PINK1 S514C antibody covalently coupled to protein G sepharose (lane 5). Immunoprecipitates were subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 S460C antibody at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.

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