COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Polyclonal Antibody - Pellino1

Name: Pellino1
Price per aliquot (100µg): £110.00
Full Name:
Pellino1 phospho Thr288 / Thr290
Long Name Text:
pellino homolog 1
Immuno Sequence:
CPVGFNT*LAFPS [residues 282 - 293 of human]
Phosphopeptide:
CPVGFNTLAFPS [residues 282 - 293 of human]
Sheep Number:
S393C
Monoclonal/Polyclonal:
Polyclonal
Gel Image:

A DNA vector encoding HA-Pellino 2 was transfected into IRAK1-null IL-1R cells with either wild-type IRAK1 (lane 1) to activate Pellino 2 (shown by a decrease in mobility using an anti-HA antibody) or with catalytically-inactive–IRAK1[K239A] (lane 2) as a control. 24 hours later the cells were lysed and 25 μg of extract protein was subjected to SDS/PAGE, transferred to nitrocellulose membrane and immunoblotted with the phospho-specific antibody that recognises phosphorylated Thr288 of Pellino 1 and the equivalent residue in Pellino 2 (Thr290). Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce). NS, nonspecific band recognised by the p-Pellino antibody. The cell extract was also immunoblotted with an anti-tubulin antibody as a loading control.

Do you need any help? Please get in touch and we’ll be happy to lend a hand.