Polyclonal Antibody - PDE3

Name: PDE3
Price per aliquot (100µg): £110.00
Full Name:
PDE3A phospho Ser 465
Long Name Text:
Phosphodiesterase 3
Immuno Sequence:
RDRSTS*IKLQEAP [residues 460 - 472 of human]
Phosphopeptide:
RDRSTSIKLQEAP [residues 460 - 472 of human]
Sheep Number:
S556B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HeLa cells growing in medium containing serum were harvested (S), or incubated with mimosine (Mim 0.2 mM for 16 h), aphidicolin (Aph 10 μg/ml for 16 h) or serum-starved for 12 hours and stimulated with PMA (TPA 400 ng/ml or forskolin (FO 20 μM). PDE3A was immunoprecipitated (IP) from 1.5 mg of each cell extract with 10 μg anti-PDE3A (1095 – 1110). The immunoprecipitates were, subjected to SDS-PAGE, transferred to a PVDF membrane and analysed by DIG-14-3-3 overlay and immunoblotted with antibodies that recognise the pSer 312, pSer 428, pSer 438, pSer 465 and pThr 568 sites on PDE3A. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1:10.000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Notes:
Include the non-phosphorylated form of the peptide immunogen at 10 μg/ml to the diluted antibody.

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