COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Polyclonal Antibody - PDE3

Name: PDE3
Price per aliquot (100µg): £110.00
Full Name:
PDE3A phospho Ser 428
Long Name Text:
Phosphodiesterase 3
Immuno Sequence:
KRLRRS*LPPGL [residues 423 - 433 of human]
KRLRRSLPPGL [residues 423 - 433 of human]
Sheep Number:
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HeLa cells growing in medium containing serum were serum starved for 12 hours and stimulated with forskolin. Where indicated, cells were incubated with H-7 (10 μM), Ro 318220 (1 μM), PD 184352 (2 μM) or no inhibitor for 1 hour, or BIRB0796 (10 μM) for 2 hours, before stimulation with PMA (400 ng/ml) for a further 15 min. PDE3A was immunoprecipitated (IP) from 3 mg of each cell extract with 20 μg anti-PDE3A (1095 – 1110). The immunoprecipitates were, subjected to SDS-PAGE, transferred to a PVDF membrane and immunoblotted with anti-PDE3A (1095 – 1110) [lower panel] or anti- PDE3A phospho Ser 428 [upper panel]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1:10.000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Include the non-phosphorylated form of the peptide immunogen at 10 μg/ml to the diluted antibody.

Do you need any help? Please get in touch and we’ll be happy to lend a hand.