PDE3

Full Name
PDE3A (1095 - 1110)
Long Name Textual
Phosphodiesterase 3
Immuno Sequence
RLAGIENQSLDQTPQS [residues 1095 - 1110 of human]
Mono/Poly clonal
Polyclonal
QC Data
Gel Image
PDE3
HeLa cells growing in medium containing serum were serum starved for 12 hours and stimulated with forskolin. Where indicated, cells were incubated with H-89 (10 μM), LY 294002 (LY, 100 μM), rapamycin (Ra, 100 nM) or U0126 (U, 10 μM) for 1 hour before stimulation with forskolin (20 μM) for a further 30 min. PDE3A was immunoprecipitated (IP) from 1.5 mg of each cell extract with 10 μg anti-PDE3A (1095 – 1110). The immunoprecipitates were, subjected to SDS-PAGE, transferred to a PVDF membrane and immunoblotted with anti-PDE3A (1095 – 1110) [lower panel] or anti-PDE3A phospho Ser 312 [upper panel]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1:10.000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

Sheep No
S721A
Aliquot
0.1mg
Price
£125.00