Polyclonal Antibody - NDRG

Name: NDRG
Price per aliquot (100µg): £100.00
Full Name:
NDRG1 [3 x Thr] phospho Thr 346, Thr 356 and Thr 366
Long Name Text:
N-myc Downstream Regulated Gene
Immuno Sequence:
CRSRSHT*SEG
Phosphopeptide:
CRSRSHTSEG
Sheep Number:
S231B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

Tissue extracts (30 μg of protein) prepared from two individual SGK1 -/- mice and two individual SGK1 +/+ mice were subjected to SDS/PAGE, transferred to ImmobilonP membranes and immunoblotted with anti-GST-NDRG1 (upper panel) or anti-NDRG1 phospho Thr x 3 (lower panel) at a concentration of 1 μg/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Notes:
Entire antigen is repeated 3 times at C-Terminus of NDRG1.

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