FESTIVE CLOSURE

Festive Closure

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Polyclonal Antibody - NCC

Name: NCC
Price per aliquot (100µg): £100.00
Full Name:
NCC phospho Ser 91
Long Name Text:
Na-Cl cotransporter
Immuno Sequence:
CTLADLHS*FLKQEGRR [residues 85 - 97 of human + N-terminal cysteine for coupling and two C-terminal arginine residues]
Phosphopeptide:
CTLADLHSFLKQEGRR [residues 85 - 97 of human + N-terminal cysteine for coupling and two C-terminal arginine residues]
Sheep Number:
S996B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HEK 293 cells were transfected with wildtype human NCC. 36 hours post-transfection, cells were treated with either basic or hyptonic low chloride medium for the specified time-points and lysed. The cell lysates were subjected to SDS-Page, followed by immunoblotting with anti-NCC phospho Thr 46 [S241C], anti-NCC phospho Thr 55 [S908B], anti-NCC phospho Thr 60 [S995B], anti-NCC phospho Ser 91 [S996B] and anti-NCC (AB3553 Chemicon) antibodies. Binding of the phospho specific primary antibodies was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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