Polyclonal Antibody - METTL1

Name: METTL1
Price per aliquot (100µg): £110.00
Full Name:
METTL1 (258 - 268)
Long Name Text:
Methyltransferase like 1
Immuno Sequence:
KQAVTPNPTLP [residues 258 - 268 of human]
Phosphopeptide:
KQAVTPNPTLP [residues 258 - 268 of human]
Sheep Number:
S177B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HEK 293 cells were serum starved for 8 hours and then incubated for 20 mins without (-) or with (+) 100 nM wortmannin before stimulation for a further 15 mins with 20 ng/ml IGF-1 or for 30 mins with 400 ng/ml PMA. The cells were lysed and METTL1 was immunoprecipitated from 1.5 mg of extract using 5 μg anti-METTL1 (S177B). The immunoprecipitate was denatured in SDS, subjected to SDS/PAGE and transferred to nitrocellulose membranes. The membranes were immunoblotted with an antibody that recognises METTL1 phosphorylated at Ser 27 (anti-METTL1 phospho Ser 27 S793A) and with an antibody that recognises the phosphorylated and unphosphorylated forms of METTL1 equally well (anti-METTL1 S177B). Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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