Polyclonal Antibody - LRRK

Name: LRRK
Price per aliquot (100µg): £110.00
Full Name:
LRRK2 phospho Ser 910
Long Name Text:
Leucine-rich repeat kinase 2
Immuno Sequence:
VKKKSNS*ISVGEFY [residues 904 - 917 of human]
Phosphopeptide:
VKKKSNSISVGEFY [residues 904 - 917 of human]
Sheep Number:
S357C
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

LRRK2 was immunoprecipitated from HEK293 cells overexpressing wildtype LRRK2, LRRK2 S910A, LRRK2 S935A and LRRK2 S910A + S935A using 5 ug of anti-LRRK2 (100 – 500) antibody per 2 mg of cellular extract. The immunoprecipitates were subjected to SDS-Page and immunoblotted with either anti-FLAG, anti-LRRK2 phospho Ser 910 at 2 ug/ml or anti-LRRK2 phospho Ser 935. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
Notes:
Include the non-phosphorylated form of the peptide immunogen at 10 μg/ml to the diluted antibody.

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