Polyclonal Antibody - IKK epsilon

Name: IKK epsilon
Price per aliquot (100µg): £100.00
Full Name:
IKK epsilon phospho Ser 172
Long Name Text:
inhibitory kB kinase epsilon
Immuno Sequence:
CEKFVS*VYGTE [residues 168 - 177 of human + N-terminal cysteine for coupling]
Phosphopeptide:
CEKFVSVYGTE [residues 168 - 177 of human + N-terminal cysteine for coupling]
Sheep Number:
S051C
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phosphorylated specific peptide.
Gel Image:

HEK 293 cells were transfected with constructs for Flag tagged wildtype and kinase dead (K38A) IKK epsilon. The transfected IKK epsilon was immunoprecipitated from cell extracts using anti-Flag M2 agarose (Sigma). The resulting immunoprecipitate was subjected to SDS-Page followed by immunoblotting with anti-FLAG (Sigma) and anti-IKK phospho Ser 172 antibodies (in the presence of non-phosphorylated or phosphorylated peptide). Binding of IKK epsilon phospho Ser 172 antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).
RAW264.7 cells were unstimulated (-) or stimulated (+) with 100 ng/ml LPS for 30 mins. 1 mg of cellular extract was subjected to immunoprecipitation using 10 ug anti-IKK epsilon phospho Ser 172 in the presence of 100 ug of non-phosphorylated peptide. The resulting immunoprecipitate was subjected to SDS-Page followed by immunoblotting with anti-TBK1 phospho Ser 172, anti-IKK phospho Ser 172, anti-TBK1 (CST) and anti- IKK epsilon (Sigma). Binding of TBK1 and IKK epsilon phospho specific antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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