(A) Lysates (1 mg) of RAW264.7 macrophages were incubated with antibodies from non-immunised sheep (IgG) or antibodies against IKK epsilon (S277C, 2nd bleed). After one hour incubation, protein G-Sepharose was added for a further 15 min. The supernatant was removed and depletion of IKK epsilon was analyzed by immmoblotting (extract). The immunoprecipitates were washed and immunoblotted (IP). NS = non- specific band (B) Lysates (1 mg) of RAW264.7 macrophages were incubated with antibodies from non-immunised sheep (IgG) or antibodies against IKK epsilon (S277C, 2nd bleed). After one hour incubation, protein G-Sepharose was added for a further 15 min. After brief centrifugation to pellet the protein G-Sepharose, the supernatant was removed and depletion of IKK eplison was analysed by immunoblotting. [Note that the related kinase TBK1 was not immunodepleted by the amtibody. Immunoblotting was performed using TBK1 and IKK epsilon antibodies purchased from Cell Signalling Technology]