Polyclonal Antibody - IKK epsilon

Name: IKK epsilon
Price per aliquot (100µg): £110.00
Full Name:
IKK epsilon (702 - 717 mouse)
Long Name Text:
inhibitory kB kinase epsilon
Immuno Sequence:
NRLIERLHRVPSAPDV [residues 702 - 717 of mouse]
Phosphopeptide:
NRLIERLHRVPSAPDV [residues 702 - 717 of mouse]
Sheep Number:
S277C
Monoclonal/Polyclonal:
Polyclonal
Gel Image:

(A) Lysates (1 mg) of RAW264.7 macrophages were incubated with antibodies from non-immunised sheep (IgG) or antibodies against IKK epsilon (S277C, 2nd bleed). After one hour incubation, protein G-Sepharose was added for a further 15 min. The supernatant was removed and depletion of IKK epsilon was analyzed by immmoblotting (extract). The immunoprecipitates were washed and immunoblotted (IP). NS = non- specific band (B) Lysates (1 mg) of RAW264.7 macrophages were incubated with antibodies from non-immunised sheep (IgG) or antibodies against IKK epsilon (S277C, 2nd bleed). After one hour incubation, protein G-Sepharose was added for a further 15 min. After brief centrifugation to pellet the protein G-Sepharose, the supernatant was removed and depletion of IKK eplison was analysed by immunoblotting. [Note that the related kinase TBK1 was not immunodepleted by the amtibody. Immunoblotting was performed using TBK1 and IKK epsilon antibodies purchased from Cell Signalling Technology]

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