Polyclonal Antibody - FUS

Name: FUS
Price per aliquot (100µg): £110.00
Full Name:
FUS phospho Thr 11
Long Name Text:
Fusion (involved in t(12;16) in malignant liposarcoma)
Immuno Sequence:
KKNDYTQQAT*QSYGAYP [ residues 4 - 19 of human]
Phosphopeptide:
KKNDYTQQATQSYGAYP [ residues 4 - 19 of human]
Sheep Number:
S781B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

Phosphorylation detected in HEK293 cells after treatment with ionising radiation (IR) (10Gy). The antibody was incubated with the non-phosphorylated peptide (40 μg/ml) for 1 hour before addition to the nitrocellulose membrane. The antibody was used at 1 μg/ml in 5 % skimmed milk / TBS-Tween and incubated with the membrane for 1 hour at room temperature. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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