Polyclonal Antibody - FOXO

Name: FOXO
Price per aliquot (100µg): £110.00
Full Name:
FOXO3a phospho Ser 294
Long Name Text:
Forkhead box O3a
Immuno Sequence:
SKWPGS*PTSR [residues 289 - 298 of human]
SKWPGSPTSR [residues 289 - 298 of human]
Sheep Number:
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

pCMV5-Flag-FOXO3a was transfected into HEK293 cells and cells were serum starved overnight and stimulated with or without arsenite (0.5 M) for 60 mins. Flag-FOXO3a was immunoprecipitated from 100 μg of cell lysate using an anti-Flag antibody (Sigma). The immunoprecipiate was split into twice, with one half treated with or without 1 mU of PP1 gamma for 30 mins at 30 °C. Samples were subjected to SDS-Page analysis before being transferred to nitrocellulose membrane and immuno blotted with the appropriate phospho specific antibody at 1 μg/ml or anti-FOXO3a (Upstate). Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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