Polyclonal Antibody - Filamin

Name: Filamin
Price per aliquot (100µg): £110.00
Full Name:
Filamin gamma phospho Ser 2213
Long Name Text:
Immuno Sequence:
GRERLGS*FGSITR [residues 2207 - 2219 of human]
GRERLGSFGSITR [residues 2207 - 2219 of human]
Sheep Number:
Purification Method:
Affinity purified against peptide.
Gel Image:

Murine C2C12 myoblasts were deprived of serum for 12 hours, then stimulated with 250 ng/ml insulin or 20 ng/ml EGF for the times indicated. The cells were lysed and the lysates subjected to SDS/Page followed by transfer to an immobilonP membrane. The membranes were then probed with anti-Filamin phospho Ser 2213 or anti-Filamin (S661B) antibodies. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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