Polyclonal Antibody - EWS

Name: EWS
Price per aliquot (100µg): £110.00
Full Name:
EWS phospho Thr 79
Long Name Text:
Ewing sarcoma RNA-binding protein
Immuno Sequence:
TGYTT*PTAPQ [residues 75 - 84 of human]
Phosphopeptide:
TGYTTPTAPQ [residues 75 - 84 of human]
Sheep Number:
S423B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified phospho specific peptide.
Gel Image:

HEK293 cells were incubated for 1 hour with (+) or without (-) 2 uM PD 184352 or 10 ?M U0126, then stimulated for 10 mins with 100 ng/ml EGF (Upper panels) or 100 ng/ml PMA (lower panels) and lysed. Cell extract (60 ug protein) was denatured, subjected on SDS/PAGE and immunoblotted with anti-EWS phospho Thr 79 (S423B) or anti-EWS (S418B). Note: that in the lower panel phorylated EWS runs just above a non-specific band as indicated.

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