Polyclonal Antibody - ERK8

Name: ERK8
Price per aliquot (100µg): £110.00
Full Name:
Long Name Text:
mitogen-activated protein kinase 8
Immuno Sequence:
His-ERK8 [DU 662]
DU Number of Antigen:
Sheep Number:
Tag Information:
Tagged with His.
Purification Method:
Affinity purified against recombinant protein.
Gel Image:

HEK 293 cells were transfected with vectors expressing either wildtype (WT) HA-ERK8 or HA-ERK8 [D154A]. At 36 hr post-transfection, the cells were exposed for 10 or 30 mins to 1 mM hydrogen peroxide, and lysed. The lysates (60 μg) were denatured by SDS, subjected to SDS-PAGE, transferred to nitrocellulose membrane and blotted with the anti-ERK8 antibody at 0.5 μg/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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