COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Polyclonal Antibody - c-Jun

Name: c-Jun
Price per aliquot (100µg): £110.00
Full Name:
c-Jun phospho Thr 239
Long Name Text:
V-jun avian sarcoma virus 17 oncogene homolog
Immuno Sequence:
CPEMPGET*PPLSPI [residues 233 - 245 of human]
Phosphopeptide:
CPEMPGETPPLSPI [residues 233 - 245 of human]
Sheep Number:
S508B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide.
Gel Image:

RAW cells were incubated with or without 2 uM PD184352 for 1 hour and/or 100 nM wortmannin for 10 mins prior to being stimulated for 30 mins with 100 ng/ml LPS and then lysed. c-Jun was immunoprecipitated from the lysates using anti-c-Jun [S230B] and the immunoprecipitates were subjected to SDS-Page and immunoblotted with anti-c-Jun phospho Thr 239 [S678A], anti-c-Jun phospho Ser 243 and anti-c-Jun [S230B] Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

Do you need any help? Please get in touch and we’ll be happy to lend a hand.