COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Polyclonal Antibody - c-Jun

Name: c-Jun
Price per aliquot (100µg): £110.00
Full Name:
c-Jun phospho Thr 93
Long Name Text:
V-jun avian sarcoma virus 17 oncogene homolog
Immuno Sequence:
NGHITTTPT*PTQFLCPK [residues 85 - 101 of human]
Phosphopeptide:
NGHITTTPTPTQFLCPK [residues 85 - 101 of human]
Sheep Number:
S679A
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide. Include the non-phosphorylated form of the peptide immunogen at 10 μg/ml to the diluted antibody.
Gel Image:

RAW cells were incubated with or without 2 uM PD184352 and/or 10 uM SB203580 for 1 hour prior to being stimulated for 30 mins with 10 ug/ml anisomycin and then lysed. c- Jun was immunoprecipitated from the lysates using anti-c-Jun [S230B] and the immunoprecipitates were subjected to SDS-Page and immunoblotted with anti-c-Jun phospho Thr 91 [S678A], anti-c-Jun phospho Thr 93 and anti-c-Jun [S230B] Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

Do you need any help? Please get in touch and we’ll be happy to lend a hand.