Polyclonal Antibody - c-Jun

Name: c-Jun
Price per aliquot (100µg): £100.00
Full Name:
c-Jun phospho Thr 91
Long Name Text:
V-jun avian sarcoma virus 17 oncogene homolog
Immuno Sequence:
NGHITTT*PTPTQFLCPK [residues 85 - 101 of human]
Phosphopeptide:
NGHITTTPTPTQFLCPK [residues 85 - 101 of human]
Sheep Number:
S678A
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho specific peptide. Include the non-phosphorylated form of the peptide immunogen at 10 μg/ml to the diluted antibody.
Gel Image:

RAW cells were incubated with or without 2 uM PD184352 and/or 10 uM SB203580 for 1 hour prior to being stimulated for 30 mins with 10 ug/ml anisomycin and then lysed. c- Jun was immunoprecipitated from the lysates using anti-c-Jun [S230B] and the immunoprecipitates were subjected to SDS-Page and immunoblotted with anti-c-Jun phospho Thr 91 [S678A], anti-c-Jun phospho Thr 93 and anti-c-Jun [S230B] Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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