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Polyclonal Antibody - AS160

Name: AS160
Price per aliquot (100µg): £110.00
Full Name:
AS160 phospho Thr 568
Long Name Text:
AKT substrate 160 kDa
Immuno Sequence:
CKAKRSLT*SSLENI [residues 562 - 574 of human]
CKAKRSLTSSLENI [residues 562 - 574 of human]
Sheep Number:
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HEK293 cells transfected with HA-AS160A were stimulated after pre-exposure to inhibitors as indicated. Crude lysates (60 μg) were subjected to SDS-Page followed by immunoblotting with anti-HA, anti-AS160 phospho Thr 568 [S028C], anti-AS160 phospho Thr 642 [S887B] and anti-AS160 phospho Ser 666 [S924B] antibodies. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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