COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Polyclonal Antibody - AS160

Name: AS160
Price per aliquot (100µg): £110.00
Full Name:
AS160 phospho Thr 568
Long Name Text:
AKT substrate 160 kDa
Immuno Sequence:
CKAKRSLT*SSLENI [residues 562 - 574 of human]
Phosphopeptide:
CKAKRSLTSSLENI [residues 562 - 574 of human]
Aliases:
TBC1D4
Sheep Number:
S028C
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HEK293 cells transfected with HA-AS160A were stimulated after pre-exposure to inhibitors as indicated. Crude lysates (60 μg) were subjected to SDS-Page followed by immunoblotting with anti-HA, anti-AS160 phospho Thr 568 [S028C], anti-AS160 phospho Thr 642 [S887B] and anti-AS160 phospho Ser 666 [S924B] antibodies. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

Do you need any help? Please get in touch and we’ll be happy to lend a hand.